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Module 4: Top-Down versus Bottom-Up Proteomics Approaches & Mass Spectrometry Primer

FACULTY

Scot R. Weinberger, President, CEO and Founder of GenNext Technologies™ Inc.

Dr. Ron Orlando, Assistant Professor Complex Carbohydrate Research Center and Departments of Biochemistry & Molecular Biology and Chemistry University of Georgia

Module four provides students with a review of top-down versus bottom-up proteomic approaches and offers a detailed primer on mass spectrometry technologies.

TOP-DOWN VS. BOTTOM-UP APPROACHES: This module provides students a review of proteomic technologies, while placing particular focus upon differentiating top-down from bottom-up approaches. Top-down approaches directly analyze nascent proteins as they present themselves in living systems, while bottom-up methodologies directly examine their proteolytic fragments. Top-down methodologies to be examined include: SELDI, 2-D gel electrophoresis; differential gel electrophoresis; virtual 2-D gel electrophoresis; hyphenated liquid chromatography (LC) combined with mass spectrometry (MS); and electrospray ionization (ESI) fourier transform ion cyclotron resonance mass spectrometry (FT ICRMS). Bottom-up methodologies to be examined include tandem peptide separation schemes, such as combined ion exchange (IEX) and RP chromatography. At the completion of this module, students will develop a strong appreciation of the fundamental differences, strengths, and weaknesses, as well as the complementary nature of bottom-up and top-down proteomic methodologies.

MASS SPECTROMETRY PRIMER: This module will provide students a primer of proteomic-based MS technologies. Lecturers will provide students with a basic understanding of how each component of an MS system functions, as well as the advantages and limitations of each process. A technical overview is provided, including detailed discussion of the primary ionization sources (ESI, MALDI, and SELDI) and MS analyzers (TOF, Q, IT, FTMS, etc.) used in proteomic studies. By participating in this module, students are expected to understand the role MS plays in proteomic studies. Additionally, students are expected to understand how to select the proper instrument for the project, and how to design experiments towards the strengths of a particular MS configuration

GenNext's 2007 In Person Translational Courses have been completed. GenNext would like to thank its Education Partners and excellent instructors for another highly received Translational Research Program.

Information regarding GenNext's 2008 program will become available shortly. Sign-up to find out about upcoming GenNext courses.

For more information about this course, please contact GenNext Technologies at inform@gennexttech.com or call 650-563-9577.