GenNext Technologies™ Inc.

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Module 7: Protein Identification and Characterization

FACULTY

Scot R. Weinberger, President, CEO and Founder of GenNext Technologies™ Inc.

Dr. Ron Orlando, Assistant Professor Complex Carbohydrate Research Center and Departments of Biochemistry & Molecular Biology and Chemistry University of Georgia

Protein identification and characterization is almost universally achieved using single or tandem mass spectrometry in combination with computational algorithms. Two fundamental approaches will be examined in this module: Peptide Mass Fingerprinting (PMF) and tandem ms analysis. High throughput (HT) PMF analysis will be reviewed using hyphenated 2-D gel MALDI MS analysis examples. Alternatively we will explore in-gel digestions for automated analysis using LC ESI MS. Students will also learn of tandem MS analysis as the gold standard for establishing peptide primary sequence, PTM, and protein identification when combined with on-line capillary RP HPLC with ESI ion trap devices. We will also explore how sequences are automatically processed and protein identification conferred using various algorithms such as SEQUEST. Other tandem MS schemes for peptide sequence determination will be reviewed including the ESI tandem quadrupole TOF MS analyzers, ESI FTICR MS, MALDI post source decay (PSD) analysis, MALDI quadrupole TOF analysis, MALDI TOF - TOF MS2 analysis, and MALDI QIT - TOF MS analysis.

This module provides students with an in-depth understanding of the need for protein identification and characterization while simultaneously instructing them in the use of modern protein identification tools, methodologies, and database mining programs. When completed, students are expected to design protein identification and characterization strategies for both top down and bottom up proteomic exercises. Furthermore, students will be instructed in the means of horizontally moving between protein and cDNA databases, allowing for the creation of directed protein discovery strategies as well as intelligent correlation of protein and genomic de novo discovery results.

GenNext's 2007 In Person Translational Courses have been completed. GenNext would like to thank its Education Partners and excellent instructors for another highly received Translational Research Program.

Information regarding GenNext's 2008 program will become available shortly. Sign-up to find out about upcoming GenNext courses.

For more information about this course, please contact GenNext Technologies at inform@gennexttech.com or call 650-563-9577.