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Module 6: A Detailed Discussion of Bottom-Up Proteomics MethodologiesFACULTY Dr. Ron Orlando, Assistant Professor Complex Carbohydrate Research Center and Departments of Biochemistry & Molecular Biology and Chemistry University of Georgia This learning module is intended to provide students with an enabling understanding of the principles of multidimensional LC-MS/MS. Included in the discussion will be issues related to chromatography, such as the interactions between sample complexity, peak capacity, dynamic range, and the selection of separation strategies. A technical overview is provided, including detailed discussion of the LC separation techniques and MS/MS parameters. Particular emphasis is given towards the design and execution of successful "bottom-up" translational proteomic studies as well as the integration of results with genomic and top-down proteomics data. Students are expected to learn the basics of multidimensional LC-MS/MS technology from this module and the role this methodology plays in bottoms-up proteomic studies. Students are further expected to be capable of designing and performing de novo biomarker discovery experiments. Additionally, students are expected to understand how to combine the results from bottom up proteomics with top-down methodologies (gel based, SELDI, and FT-MS) as well as gene array expression analysis.GenNext's 2007 In Person Translational Courses have been completed. GenNext would like to thank its Education Partners and excellent instructors for another highly received Translational Research Program.Information regarding GenNext's 2008 program will become available shortly. Sign-up to find out about upcoming GenNext courses. For more information about this course, please contact GenNext Technologies at inform@gennexttech.com or call 650-563-9577.
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